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Friday, July 24, 2020 | History

3 edition of An analysis of the genes encoded in the Us region of HSV-1 found in the catalog.

An analysis of the genes encoded in the Us region of HSV-1

Richard Mayne Longnecker

An analysis of the genes encoded in the Us region of HSV-1

by Richard Mayne Longnecker

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  • 38 Currently reading

Published .
Written in English


Edition Notes

Statementby Richard Mayne Longnecker.
Classifications
LC ClassificationsMicrofilm 88/225 (Q)
The Physical Object
FormatMicroform
Paginationix, 118 p.
Number of Pages118
ID Numbers
Open LibraryOL2161120M
LC Control Number88890025

The gene designated {gamma}{sub 1} maps in the inverted repeats flanking the long unique sequence of herpes simplex virus-1 (HSV-1) DNA, and therefore it is present in two copies per genome. This gene is not essential for viral growth in cell culture. Four recombinant viruses were genetically engineered to test the function of this gene. A. Herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2) can be distinquished by their antigenicity and in general the location of the lesions they produce. Lesions caused by HSV-1 are generally above the waist, and those of HSV-2 are below the waist.

@article{osti_, title = {Expression of varicella-zoster virus and herpes simplex virus in normal human trigeminal ganglia}, author = {Vafai, A and Wellish, M and Devlin, M and Gilden, D H and Murray, R S}, abstractNote = {Lysates of radiolabeled explants from four human trigeminal ganglia were immunoprecipitated with antibodies to varicella-zoster virus (VZV) and to herpes simplex virus. 8 Functional analysis of HSV-1 receptors HveA and HveC on CD34+ Cells. 57 9 HveA and HveC receptor analysis on infected CD34+ cells. 59 10 Analysis of rhEPO production from CD34+ cells transduced with HSV-1 vector DHEPO at various MOI. 61 11 Analysis of QOZHG transduced CD34+ cells for one week in culture. 63File Size: 6MB.

  Have you ever wondered what the real difference is between herpes simplex 2 (HSV-2) and herpes simplex 1 (HSV-1)? I have spent the past four years as the educational coordinator for the Colorado H Club answering questions like this every day. Genetically speaking, the two viruses are about 85% the same and the symptoms they produce are exactly the same too. The pathogenic WT HSV-1 strain 8F (Yu et al., ) and the mutant M1 strain (Xu et al., b) were used in our experiments. The virus titers were determined using the 50% tissue culture infectious dose (TCID 50) method with Vero cells. CRISPR/Cas9 g-RNA design of the HSV-1 UL41 and LAT genesCited by: 6.


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An analysis of the genes encoded in the Us region of HSV-1 by Richard Mayne Longnecker Download PDF EPUB FB2

In this report we identified a novel HSV-1 transcript, expressed from the unique-short region of the viral genome. It spans the region from the U S 5 to the α22 genes and is transcribed antisense to U S 5, U S 4, U S 3 and α22 mRNAs, with γ2 kinetics. Our data suggest that U S is a long non-coding RNA.

The role of this transcript is Cited by: 2. Herpes simplex virus 1 and 2 (HSV-1 and HSV-2), also known by their taxonomical names Human alphaherpesvirus 1 and Human alphaherpesvirus 2, are two members of the human Herpesviridae family, a set of new viruses that produce viral infections in the majority of humans.

Both HSV-1 (which produces most cold sores) and HSV-2 (which produces most genital herpes) are common and : incertae sedis. However, for four polypeptides predicted for HSV-1 Us genes, clear homologues were found in four predicted polypeptide species encoded in the Us region of the distantly related herpes virus, varicella-zoster virus (Davison, ; also, unpublished results), and this is Cited by:   A kbpBam HI restriction fragment representing most of the unique short (Us) region of the genome of the simian α-herpesvirus SA 8 was identified and cloned.

Partial sequencing of this DNA fragment identified regions of sequence homology with eight open reading frames (ORFs) of HSV 1 and/or HSV 2. Sequence and size analysis of subcloned fragments of the SA 8 Us region and Cited by: Abstract. We have constructed a map of the genes encoded by a 23,nucleotide-pair region of herpes simplex virus type 1.

This region, defined by the three adjacent EcoRI fragments N (map coordinates to ), F ( to ), and M ( to ), has previously been shown by genetic analysis to contain the genes for thymidine kinase, nucleocapsid protein p40, glycoprotein B Cited by: The HSV-1 genome is a linear, double stranded DNA duplexbase pairs in length, and with a base composition of 67% G + C.

The genome circularizes upon infection. Because the genome circularizes, the transcription and genetic map is conveniently shown as a circle. The HSV-1 genome has an architecture composed of a unique long (UL) element, a unique short (US) element, and two distinct inverted repeat elements which flank the UL and US termed the repeat long (RL) and repeat short (RS) ().A total ofsequence reads were generated from GS FLX+ system with the sequence length ranging from 40 bp to bp with mean sequence length at bp Cited by:   The genome of herpes simplex virus 1 encodes at least 84 transcripts from which proteins are translated and several additional RNAs whose status as mRNAs is unknown.

These RNAs include latency-associated transcript, OriS1 and OriS2 RNAs and in case of α4 null mutant additional transcript that spans the junction between L and S component of the HSV-1 by: 2.

To capture the full complexity of HHV-6A and HHV-6B genomes, we applied next generation sequencing methods that map genome-wide RNA expression and translation to HSB-2 and Molt-3 cells infected for 72 hr with HHV-6A strain GS and HHV-6B strain Z29, respectively ().For each virus we mapped genome-wide translation events by preparing three different ribosome-profiling Cited by: 2.

In the present study, we determined the sequence diversity of the complete genes coding for glycoproteins G (gG), I (gI), and E (gE), comprising % of the HSV-1 genome and located within the. The pathogenesis and severity of HSK is largely determined by an interaction between viral genes encoded by the strain of HSV-1 and the make up of the host's immune system.

In this respect, mutagenesis and sequence analysis of genes encoded by the virus are of great importance. However, the herpesvirus genomes are large, with sizes ranging between and over kbp and encoding between 70 and genes (see ref. 1Author: Niels de Wind, Maddy van Zijl, Anton Berns.

F1: Map of HSV-1 genome and schematic representation of replication-competent and defective vectors. A schematic representation ofthe position of the genes encoding proteins involved in virus replication, regulation, in virus formation and assembly, and in virion structuralproteins is depicted.

The HSVencoded primosome consists of three subunits with molecular masses of 98, 79, andDa, the products of the UL5, UL8, andUL52 genes, respectively. The holoenzyme consists of a association of the UL5, UL8, and UL52 gene products, with a native molecular mass of ∼ kDa (37, 38).

The influence of genetic properties of parts of the HSV-1 genome on suppression of humoral antibody formation was investigated by using intratypic recombinants. The deleted strain HFEM (HSV-1) induces suppression.

The MluI DNA fragment (coordinates – m.u.) derived from the antibody inducing strain F1 (HSV-1) was transfected into the deleted strain HFEM to produce the Cited by: 4. Exploring how herpes simplex virus changes when passed between family members Date: Octo Source: Penn State Summary: A new study offers a.

A deubiquitinating enzyme encoded by HSV-1 belongs to a family of cysteine proteases that is conserved across the family Herpesviridae.

Mol Cell Kawaguchi, Y., K. Kato, M. Tanaka, M. Kanamori, Y. Nishiyama, and Y. Yamanashi. Conserved protein kinases encoded by herpesviruses and cellular protein kinase cdc2 target the same. Microarray analysis of host gene expression for comparison between naïve and HSV-1 latent rabbit trigeminal ganglia Christian Clement,1 Michael P.

Popp,3 David C. Bloom,4,5 Gregory Schultz,5,6 Li Liu,3,6 Donna M. Neumann,1 Partha S. Bhattacharjee,1 James M. Hill1,2 1Department of Ophthalmology, New Orleans, LA; 2Departments of Pharmacology, Microbiology and Neuroscience. Nucleotide sequences of the region encompassing reiteration VII of HSV (a) Nucleotide sequences of strain 17 (as the standard) and isolate Ty2.

The nucleotide numbering system used is the short unique region of strain 17 (McGeoch et al. ).Nucleotide sequences recognized by restriction endonuclease BamHI were single copy of the bp tandem repeats of reiteration VII Cited by: 2.

HSV-1 has a large double stranded DNA genome of approximately kb that is composed of a unique long (UL) region ( kb), a unique short (US) region ( kb), two copies of a long inverted repeat (RL) ( kb each), and two copies of a short inverted repeat (RS) ( kb each).

During primary infection, HSV-1 undergoes productive repli-File Size: KB. HSVencoded miR-H27 inhibits a cellular transcriptional repressor of viral IE and early genes by blocking the expression of KLHL24, evading host cell defenses, and supporting the efficient replication.

HSVencoded miR-H28 and miR-H29 were expressed late in productive infection and exported from infected cells via : Yongzhong Duan, Jieyuan Zeng, Shengtao Fan, Yun Liao, Min Feng, Lichun Wang, Ying Zhang, Qihan Li.The HSV-1 helicase-primase is a trimeric complex consisting of the products from UL5, UL8 and UL52 genes.

These genes are essential for HSV-1 DNA replication and perform many functions during the viral DNA replication process. UL5 is believed to be the helicase based on the presence of seven conserved helicase motifs, UL8 has no known enzymatic activities, while UL52 is believed to be the Author: Stacy Dawn Carrington-Lawrence.

Researchers have identified a region on the long arm of human chromosome 21 with high odds--at least 1,toof being linked to cold sore susceptibility.